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Rice
Figure 3 | Rice

Figure 3

From: Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage

Figure 3

Physical interaction of OsUbc13 with Mms2/Uev1A from other species. a Physical interaction between OsUbc13 and human Mms2/Uev1A in a yeast two-hybrid assay. b Physical interaction between OsUbc13 and yeast Mms2 in a yeast two-hybrid assay. a,b The PJ69-4A transformants carrying one Gal4AD (from pGAD424 derivative) and one Gal4BD (from pGBT9 derivative) were replicated onto various plates as indicated and incubated for 3 days before being photographed. The result is representative of at least five independent transformants from each treatment. c Protein interactions between OsUbc13 and human Mms2/Uev1A by an affinity pull-down assay. A crude whole-cell extract from co-transformed E. coli cells was incubated with 100 μl GST beads for 2 hr at 4°C. The beads were then boiled with SDS-PAGE loading buffer for 5 min before SDS-PAGE analysis. GST-hUev1A (Lane 1), GST-hMms2 (Lane 3) , GST (Lane 5) and His-OsUbc13 (Lane 7) alone served as controls. pGEX6 (GST) co-transformed with His-OsUbc13 (Lane 6) also served as a negative control.

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