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Figure 2 | Rice

Figure 2

From: Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage

Figure 2

Functional complementation of yeast ubc13 null mutant by OsUBC13. a Protection of the ubc13 mutant by OsUBC13 from MMS-induced DNA damage by a liquid killing experiment. Cells were grown in`rich medium 30 min prior to the treatment. Results are from the average of at least three independent experiments with standard deviations. (■) HK578-10D (wild type); (▴) WXY904 (ucb13) and (○) WX904 transformed with pGAD-OsUbc13. b Protection of the ubc13 mutant by OsUBC13 from representative DNA-damaging agents by a serial dilution assay. Cells were grown overnight in SD selective media, diluted and treated as previously described (Wang et al. [2011]). Photographs were taken after 2-day incubation at 30°C.

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