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Rice
Figure 5 | Rice

Figure 5

From: An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves

Figure 5

Analysis of OsSLR1 silencing transitivity in agro infiltrated leaf tissues. (A) Schematic representation of the endogenous OsSLR1 gene with size and position of the different probes used to detect primary and secondary siRNAs. (B) Time-course analysis of primary (GST probe) and secondary (5’ and 3’ probes) OsSLR1 siRNA after rice leave agroinfection. (C) Sequences of potential siRNAs detected with Ambion software “siRNAs Target Finder” from 5’, GST and 3’ sequence part of OsSLR1 coding sequence. (D) Time-course analysis of primary (putative siRNAs mix from GST as probe) and secondary (putative siRNAs mix from 5’ and 3’ part of OsSLR1 coding sequence as probes) OsSLR1 siRNA following rice leaves agroinfection and in OsSLR1 RNAi mutant leaves (M).

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