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Rice
Figure 4 | Rice

Figure 4

From: An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves

Figure 4

Analysis of the agroinfiltrated leaf tissues. (A) Accumulation of OsPDS and OsSLR1 mRNAs revealed by RT-PCR during the time course experiment. Ef1α mRNA was used as a standard. (B) Time course of OsPDS and OsSLR1 siRNAs accumulation following leaf agroinfection. Detection of siRNAs was performed using a GST probe. Arrow indicates the 21-nt-size-class siRNAs. Fifteen μg of total RNA extracted from leaf tissues after 2, 4, 8, 12 and 20 days were loaded on gel. A parallel time course was achieved with leaf tissues of plants agro-infiltrated with the pBIOS738 empty vector that served as reference value for siRNA accumulation. (C) Quantification of siRNAs detected by northern blotting using the ImageQuant™ TL image analysis software.

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