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Figure 3 | Rice

Figure 3

From: An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves

Figure 3

Analysis of HpRNA T-DNA stable transformants. (A) Northern blot detection of OsPDS and OsSLR1 mRNA accumulation in different tissues of wild type plants of cv. Nipponbare. (B) Phenotypes of RNAi mutant lines expressing OsPDS RNAi HpRNA construct. (C) Detection of 21-nts siRNAs in OsPDS and OsSLR1 HpRNA transgenic lines exhibiting or not a mutant phenotype. A probe specific to the gene specific tag (GST) and a probe representing regions extending 3’ and 5’ from the GST interval have been sequentially used. L (leaf), R (root), Nb (Control Nipponbare plant).

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