Figure 2

Agroinfection of rice leaf tissues with A. tumefaciens suspension. Histochemical assays to assess the expression of the uidA gene in rice tissues were carried out by staining with 5-bromo-4-chloro-3-indolyl-beta-D-glucuronid acid as described by Jefferson (1987). (A) Schematic representation of the small apparatus carrying many needles used to wound rice leaves. (B) Wounded rice leaves soaking in the Agrobacterium suspension in containment greenhouse. (C) GUS staining observed after 1, 3, 6 and 10 days post agroinfection (d.p.a) for three different cultivars of rice. No GUS staining was observed in unwounded and soaked leaves and in wounded but unsoaked leaves (not shown).