Figure 1From: An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leavesSchematic maps of the pBIOS738 (W. Paul, Biogemma) and pC5300-UGN T-DNAs. (A) The inverted repeat gateway cassette cloned between the O. sativa tubulin intron is under the control of the strong constitutive promoter of the cassava vein mosaic virus (CsVMV). Sc4A: promoter of the fourth subunit of the subterranean clover mosaic virus. nptII: neomycin phospho transferase II: Tnos : terminator of the Agrobacterium nopaline synthase gene: tCaMV : 3’ sequence of the Cauliflower Mosaic Virus (B) The core structure of the pC5300-UGN T-DNA. pUbi: promoter of the maize polyubiquitin 1 gene. uidA: E. coli β-glucuronidase coding sequence.Back to article page