Figure 2

Generation of the EXG1 overexpressing plants. a and b Expression of EXG1 in the Pubi-EXG1 transgenic plants. RNAs isolated from leaves of the self-progenies of the Pubi-EXG1 primary transformants (Nipponbare in a and Taichung 65 in b) and the vector-transformed control plant were reverse-transcribed with the oligo(dT) primer and amplified by EXG1 or actin specific primers. RT– indicates that reverse-transcriptase was omitted from the reaction mixture. c Cellulase activities of the Pubi-EXG plants. Protein extracts prepared from transgenic leaf blade of young seedlings were incubated with a fluorescent substrate 4-methylumbelliferyl β-D-cellobioside. v: a vector-transformed control plant.