Fig. 6From: Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)Schematic presentation of the stress treatments applied before sampling spikelets for protein expression study in experiment 1 (a) and flag-leaf% relative water content (RWC) used as a measure to determine the intensity of water stress in both water-deficit (WD) and combined-stress treatment (b). In experiment 3, heat stress and controlled water-deficit stress were imposed on the starting day of anthesis till the end of main tiller anthesis (c) and the flag-leaf RWC during the period was continuously monitored for four consecutive flowering days (day 1 [D1], day 2 [D2], day 3 [D3], and day 4 [D4]) (d). Duration from the start of stress till the flag-leaf RWC attained the target water content on day 1 (D1) was 5 days and the rewatering data were collected 3 days after rewatering (indicated by parallel line breaks in d).Back to article page