Fig. 2
From: Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)
A master gel from 2D electrophoresis using 4–7 pH IPG strips showing all 29 differentially expressed spikelet proteins (white arrowheads) under exposure to water deficit, heat, and/or combined stress. First-dimensional focusing (IEF) was done by using 17-cm IPG strips with a pH 4–7 loaded with 100 μg of total spikelet protein. In the second-dimension SDS-PAGE, 12% gels were used. Experimental molecular weight was fixed by internal markers identified earlier with the sample and indicated by Mr.