Fig. 4

The genetic relationships between seven Pokkali accessions (indicated by their IRGC accession numbers) and IR29, FL478, NIL-17, and NIL-30 are shown in (a) using 17 Saltol markers, and in (b) using 61 background SSR markers. Two gel pictures are provided as examples of multiple Pokkali alleles in (c) with marker RM8094 and in (d) with marker RM1287 for 24 DNA samples: lane 1 Nipponbare; lanes 2–8 Pokkali IRGC 8948, IRGC 15388, IRGC 15602, IRGC 15661, IRGC 28609, IRGC 26869, and IRGC 108921; lanes 11 and 12 IR29; lanes 13 and 14 FL478; lane 16 FL378; lanes 17–21 other RILs; and lanes 22–24 other NILs. A 25-bp DNA ladder is shown flanking the lanes, with sizes of 300, 275, 250, 225, 200, 175, and 150 bp from top to bottom for panel (c) and starting with 275 bp for panel (d). For marker RM8094, the FL478 allele appears to match the Pokkali accession IRGC 28609 at approximately 165 bp (c), while for RM1287, FL478 is monomorphic with IR29, but FL378 appears to match several Pokkali accessions at approximately 175 bp (d).