Fig. 3

Substitution mapping of QTLs affecting SPAD value in 39 CSSLs derived from a cross between Sasanishiki and Habataki. a (left) Graphical genotypes of the 39 CSSLs, Sasanishiki, and Habataki (Ando et al. 2008). Black denotes regions homozygous for Habataki alleles, white denotes regions homozygous for Sasanishiki alleles, and gray denotes heterozygous regions. (right) SPAD values corresponding to each graphical genotype. Bars indicate means, and error bars indicate SD. Black indicates a significant difference from Sasanishiki at the 1% level by Dunnett’s test. b Chromosome region affecting SPAD value (shown by double-headed arrow) on chromosome 4, predicted from the difference in SPAD values between each CSSL and Sasanishiki. **Significant difference from Sasanishiki at the 1% level by Dunnett’s test.