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Fig. 4 | Rice

Fig. 4

From: OsWRKY IIa Transcription Factors Modulate Rice Innate Immunity

Fig. 4

Xoo inoculation results and RNA analysis of WRKYssRNAi progeny. a Leaf lesion lengths of WRKYssRNAi T2 segregating progeny. Kitaake plants were used as controls. Leaves were inoculated when the plants were 5 weeks old; lesion lengths were measured 14 days afterwards. Each bar represents the average and standard deviation of at least three leaves. b A picture of lesions developed on leaves from T1 progeny of WRKYssRNAi line 26 and Kitaake taken 14 days after Xoo inoculation. c Xoo growth curve analysis of progeny of WRKYssRNAi-26-6 and Kitaake lines. For each time point, the bacterial population size was determined separately for three leaves of similar growth stage from three plants. Error bars represents the standard deviation of three leaves. d RNA expression levels of OsWRKY IIa genes in un-inoculated leaves of WRKYssRNAi T2 progeny. RT-PCR was performed using gene-specific primers targeted to the regions not represented in the WRKYssRNAi construct. 18S ribosomal RNA was used as an internal control.

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