Fig. 1

Schemetic representation of the WRKYssRNAi construct. a The structure of the WRKYssRNAi construct. The OsWRKY IIa sequences (OsWRKY62, 228 bp; OsWRKY71, 265 bp; OsWRKY28, 186 bp; OsWRKY76, 165 bp) were placed in the antisense-GUS linker-sense orientation. b Diagrams of the OsWRKY IIa coding regions. Regions used to generate the WRKYssRNAi construct are labeled by diagonal lines. The grey boxes indicate the regions which were amplified by RT-PCR using the gene specific primers for endogenous gene expression analysis.