Fig. 7From: Rice Germin-Like Proteins: Allelic Diversity and Relationships to Early Stress ResponsesHydrogen peroxide (H2O2) accumulation in leaf tissue of resistant and susceptible cultivars after inoculation with M. oryzae isolate P06-6. H2O2 was visualized using 3,3′-diaminobenzidine (DAB); oxidation of DAB by H2O2 results in a dark brown color (dark spots on image). Twenty-one-day-old plants of highly resistant cultivar, SHZ-2, and susceptible cultivar, LTH, were spray-inoculated with M. oryzae, and leaves were collected at 0, 12, 24, and 48 hpi. Leaves of SHZ-2 showed a higher number of DAB stained spots at 24 and 48 hpi compared to LTH.Back to article page