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Fig. 6 | Rice

Fig. 6

From: Rice Germin-Like Proteins: Allelic Diversity and Relationships to Early Stress Responses

Fig. 6

Subcellular localization of OsGLP8-6 and OsGLP8-12. a Each GLP protein was fused with a C′GFP tag and co-expressed with a golgi-specific marker protein with an mCherry tag (Nelson et al. 2007) in N. benthamiana via A. tumefaciens transformation. Epidermal peels were examined by fluorescence microscopy 4 days post-inoculation with A. tumefaciens strains containing the constructs and images are shown at ×400 magnification. Both GLP proteins show bright punctate patterns in green channel images. In some cases, GLP proteins co-localized with golgi marker proteins at cell margins (merged images). However, GLP proteins also showed distinct localizations in several planes of view across cell surfaces. b Fusion proteins were detected by immunoblot with anti-GFP antibodies. Both show expected sizes around 56 kDa compared to GFP expressed in M. oryzae at 26 kDa (Kankanala et al. 2007). OsGLP12+C′GFP showed two bands indicating multiple isoforms.

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