Fig. 4

Validation of MPSS data using RT-PCR and SBS analyses. a Validation of the MPSS tags identified in PLA and PLW using RT-PCR analysis. The RNA isolated from leaves of plants that were infested with beet armyworm (1), infested with water weevil (2), mechanically wounded (3), or unwounded (4) was used. M 1-kb size ladder. Ubiquitin gene was amplified as a loading control. b Commonly and specifically expressed genes in PLW (MPSS) and SPLW (SBS). The distinct expressed genes identified in the SBS and MPSS libraries were used in the analysis. c Correlation of the gene expression patterns between the MPSS and SBS libraries. The genome-matched reliable and significant signatures from both MPSS and SBS libraries were subjected to Pearson’s correlation analysis. Sixteen outliers that affected the correlation were removed based on regression analysis as described in Gowda et al. (2006).