Treatmentsa
| % of infected leaves (no. of infected leaves/total no. of leaves)b
| Mean lesion length of leaves showing disease symptoms (cm) ± SDc
|
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Exp 1 | Exp 2 | Exp 3 | Exp 4 | Exp 1 | Exp 2 | Exp 3 | Exp 4 |
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Water | 100 (19/19) | 100 (28/28) | 100 (21/21) | 100 (25/25) | 11.2 ± 7.01 | 10.50 ± 6.42 | 11.86 ± 4.82 | 10.18 ± 3.66 |
LBA4404/ pSB11 | 72 (18/25) | 75 (21/28) | 77 (14/18) | 76 (19/25) | 10.8 ± 7.36 | 9.38 ± 5.93 | 7.71 ± 5.30 | 8.68 ± 5.75 |
LBA4404/ pOsAP2/ERF
| 30 (7/23) | 50 (10/20) | 50 (9/18) | 48 (12/25) | 3.57 ± 1.51 | 5.0 ± 1.56 | 3.56 ± 1.24 | 5.58 ± 1.73 |
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aMidveins of leaves of 40Â days old rice plants (TN-1) were preinjected with either water alone, or water containing ~109 cells of LBA4404/pSB11 or LBA4404/pOsAP2/ERF. After 6Â h, Xanthomonas oryzae pv. oryzae was inoculated onto the midvein, 2 to 3Â cm below the point of initial injection, by pricking with a needle that had been used to touch a freshly grown colony of Xanthomonas oryzae pv. oryzae. Lesion lengths were measured 10Â days after inoculation
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b18–28 leaves were used in each experiment and four independent replications were performed
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cLesion lengths were measured 10 days after inoculation. Mean and standard deviation were calculated for each of the treatments. ANOVA was performed and the values obtained after treatment with LBA4404/pOsAP2/ERF were found to be significantly different (p < 0.05) as compared to all other treatments