Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Rice

Table 5 Transient Transfer of OsAP2/ERF Induces Callose Deposition in Rice Leaves

Mean number of callose deposits/0.6 mm² area ± SD^a
Exp^b	Water	Cellulase	LBA4404/pSB11	LBA4404/pOsAP2/ERF
1	13.30 ± 4.08	141.20 ± 37.43	23.10 ± 10.07	87.00 ± 15.29
2	11.70 ± 3.09	154.00 ± 37.21	29.00 ± 15.81	97.50 ± 35.73
3	14.40 ± 3.50	126.10 ± 29.17	25.50 ± 6.43	80.00 ± 16.47

^aRice leaves were syringe infiltrated with one of the following: water, cellulase (100 µg ml⁻¹), LBA4404/pSB11, and LBA4404/pOsAP2/ERF, stained with aniline blue, and examined under epifluorescence microscope. Mean and standard deviation were calculated for number of callose deposits from a leaf area of 0.60 mm²
^bData were collected from at least ten leaves in each experiment. ANOVA was performed, and the values obtained after infiltration with LBA4404/pOsAP2/ERF were found to be significantly different (p < 0.05) as compared to all other treatments