Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Rice

Table 4 Suppression of Cellulase-induced Expression of OsRERJ1 and OsAP2/ERF Transcription Factor Genes by Wild-type X. oryzae pv. oryzae

Genes^a	Experiment-1^b		Experiment-2^b		Experiment-3^b
Genes^a	ClsA^c	ClsA + Xoo^c	ClsA^c	ClsA + Xoo^c	ClsA^c	ClsA + Xoo^c
OsAP2/ERF	5.56	1.33	5.11	1.43	2.64	0.66
OsRERJ1	8.26	1.48	9.45	1.7	5.86	1.36

^aRice genes upregulated following cellulase treatment
^bFifteen days old rice seedlings were infiltrated with either cellulase (ClsA) or cellulase along with wild type X. oryzae pv. oryzae (ClsA + Xoo). Buffer-treated leaves were used as control. Total RNA was isolated from each of the samples, converted into cDNA and subjected to real-time PCR analysis
^cThe relative fold changes were calculated by 2^–ddCT method in comparison to expression in buffer treated leaves. The OsGAPDH (Glyceraldehyde-3-phosphate dehydro-genase) gene was used as an endogenous control