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Fig. 3 | Rice

Fig. 3

From: Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Fig. 3

Transient transfer of OsAP2/ERF induces callose deposition in rice leaves and cell death in rice roots. Rice leaves were syringe infiltrated with one of the following: water, ClsA, LBA4404/ pSB11, and LBA4404/pOsAP2/ERF. After 15 h, they were stained with aniline blue and examined under epifluorescence microscope. White spots in these pictures are indicative of callose deposition. b cellulase and d LBA4404/pOsAP2/ERF induce increased callose deposition compared with either a water or c LBA4404/pSB11. Rice roots were also treated either with water, cellulase, LBA4404/pSB11, LBA4404/pOsAP2/ERF and stained with propidium iodide (PI), and examined under a confocal microscope. Treatment with either water (e) or LBA4404/pSB11 (g) did not induce cell death (intake of PI), whereas treatment with cellulase (f) and LBA4404/pOsAP2/ERF (h) induced cell death and extensive nuclear fragmentation. Similar results were obtained in at least three independent experiments.

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