Fig. 2
Real-time PCR analysis of some differentially expressed genes. Total RNA was isolated from either ClsA- or buffer-treated rice leaves; first-strand cDNA was synthesized, and gene expression was assayed by SYBR green-based real-time PCR. The genes that were assayed are the following: OsAP2 (AP2/ERF transcription factor), OsRERJ1 (methyl JA induced helix loop helix rice transcription factor), OsOPR2 (a 12-oxophytodienoate reductase), OsMYC (a key transcription factor in the JA-mediated defense pathway), OsAOS (Allene Oxide Synthase), OsCOPINE1, OsSGT (salicylic acid glucosyltransferase), OsBSMT1 (benzothiadiazole-induced S-adenosyl-L methionine:salicylic acid carboxyl methyltransferase1), and OsMtN3 (Os8N3; a host susceptibility factor). The relative fold changes were calculated by using 2-ddCT method using the expression of buffer treated leaves as a comparison. The OsGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) gene was used as an endogenous control. Mean and standard deviation from at least four biological replicates for each gene are presented.