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Rice

Table 3 Comparison of Microarray and qPCR Results in Selected Groups of Significant Genes

From: Stress Response Versus Stress Tolerance: A Transcriptome Analysis of Two Rice Lines Contrasting in Tolerance to Phosphorus Deficiency

TIGR5 locus

Shoot

Root

MAsG

MAsE

qPCR-sG

qPCR-sE

MArG

MA-rE

qPCR-rG

qPCR-rE

Os02g31030

 

3.6

 

15.4

 

3.2

 

1.9

Os01g04920

 

4.4

 

3.0

 

4.1

 

3.4

Os01g56880

 

3.3

 

3.1

 

3.7

 

2.4

Os10g30790

 

2.2

 

18.4

 

2.6

 

1.9

Os01g52230

−1.2

2.8

−1.6

2.3

 

7.1

 

1.6

Os04g10750

−1.9

 

−1.7

  

2.5

 

3.4

Os02g56310

 

2.8

 

5.1

    

Os05g41480

 

1.7

 

2.9

    

Os04g41680

 

6.5

 

2.6

    

AK108907

 

6.1

 

3.4

−1.7

 

−1.1

 

Os11g33270

    

2.4

 

4.5

 

Os04g51460

    

2.3

 

0.6

 

Os06g48160

    

2.2

 

3.0

 

Os10g02390

 

0.4

 

0.5

6.4

 

4.9

 
  1. Data are presented as relative comparison (−P/+P) or (NIL6-4/Nipponbare). Data are the means of three independent replications. Effects highlighted in italic print are significant based on two-factorial ANOVAs conducted separately for array and qPCR data at confidence levels p ≤ 0.001 (array) or p ≤ 0.05 (qPCR)
  2. MAsG microarray genotype effect, MAsE microarray environment effect, Os02g31030 glycerophosphodiester phosphodiesterase, Os01g04920: glycosyl transferase, Os01g56880 purple acid phosphatase, Os10g30790 inorganic phosphate transporter 1–4, Os01g52230 phosphatase phospho1, Os04g10750 inorganic phosphate transporter 1–5, Os02g56310: protein kinase, Os05g41480 plastidic phosphate, Os04g41680 endochitinase A, AK108907 Os high-affinity phosphate transporter 2, Os11g33270, Os04g51460, and Os06g48160 xyloglucan endotransglucosylase/hydrolase, Os10g02390 NAD(P)H-dependent oxidoreductase
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