Fe and Zn concentrations in polished T2 seeds and leaves obtained from soil culture with slow-release fertilizer. Ten well-polished seeds obtained from the main tillers of six plants in each of six T1 sublines were analyzed. a Fe concentration. b Zn concentration. c Fe contents per seed. d Zn contents per seed. The data shown for the NT line represent the mean ± standard error for six plants cultivated in independent pots (a–d). e Fe concentration in leaves. f Zn concentration in leaves. After T2 seeds were harvested, leaf sheaths and leaf blades were also harvested and Fe and Zn concentration were measured. The data shown for each line represent the mean ± standard error for three replication of sample digestion (e, f).