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Fig. 2 | Rice

Fig. 2

From: Overexpression of the Barley Nicotianamine Synthase Gene HvNAS1 Increases Iron and Zinc Concentrations in Rice Grains

Fig. 2

Northern blot analysis of HvNAS1 gene expression in transgenic lines. The HvNAS1 ORF was used as a probe. Total RNA was extracted from the shoots of 44-day-old plants grown hydroponically under Fe-sufficient conditions, and 20 μg of total RNA was loaded into each lane. Ethidium bromide staining was used to ensure equal loading of rRNA. NT non-transgenic line; 35S, 35S-HvNAS1 transgenic line; AN1, AN2, and AN3, OsActin1-HvNAS1 transgenic lines.

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