Fig. 3
From: Transgene Stacking and Coordinated Expression of Plant Defensins Confer Fungal Resistance in Rice
Northern blot analysis of transgenic lines for the expression of rice OsPR-1a gene. About 20 µg/sample of total RNA were separated in a formaldehyde/agarose gel, transferred onto nylon membranes and hybridized to a non-radioactively labeled PR1a probe; lane 1 control, non-infected wild-type plants; lane 2 DR-T-6; lane 3 DR-T-4; lane 4 DR-T-11; lane 5 M. oryzae-infected untransformed Pusa basmati 1. b rRNA bands (28 and 18S) from an ethidium bromide-stained gel of the corresponding RNA samples indicate equivalent loading of RNA.