Fig. 2
From: Transgene Stacking and Coordinated Expression of Plant Defensins Confer Fungal Resistance in Rice
a Northern blot analysis in leaves of transgenic T2 generation lines transformed with constructs, pUb1-DR-T, pUb1-D-T, and pUb1-R-T; lane 1 Dm-T-32, lane 2 Dm-T-14, lane 3 negative control (untransformed Pusa basmati 1), lane 4 DR-T-4, lane 5 DR-T-22, lane 6 DR-T-6, lane 7 negative control (untransformed Pusa basmati 1), lane 8 Rs-T-42, lane 9 Rs-T-24. About 20 µg/sample of total RNA were separated in a formaldehyde/agarose gel, transferred onto nylon membranes and hybridized to a non-radioactively labeled probe from the coding region of chimeric polyprotein gene (Dm-AMP1+Rs-AFP2). C Negative control (untransformed rice plants); pUb1-D-T rice plants transformed with Dm-AMP1; pUb1-R-T Rice plants transformed with Rs-AFP2; pUb1-DR-T rice plants transformed with the polyprotein construct. Actin was used as internal control. rRNA bands (28 and 18S) from an ethidium bromide-stained gel of the corresponding RNA samples indicate equivalent loading of RNA. b Immunodetection of Dm-AMP1 in transgenic rice lines transformed with polyprotein construct; lane 1 pre-stained marker (CPM1); lane 2 DR-T-22; lane 3 DR-T-6; lane 4 DR-T-4; lane 5 DR-T-11; lane 6 Dm-T-32 (positive control); lane 7 negative control (untransformed Pusa basmati 1). c Immunodetection of Rs-AFP2 in transgenic rice lines transformed with polyprotein construct; lane 1 DR-T-22; lane 2 DR-T-6; lane 3 DR-T-4; lane 4 DR-T-11; lane 5 Rs-T-42 (positive control); lane 6 negative control (untransformed Pusa basmati 1).