Co-segregation of resistance phenotype and AK102429 gene in G978 × IR64 BC2F2 progeny. a
BamHI restriction sites in relation to the gene structure of AK102429 on chromosome 12 and the position of probe used for Southern blot. b Southern blot analysis of G978 × IR64 BC2F2 progeny (17 resistant and seven susceptible) using AK102429 gene probe defined by the primer pair: UboxMF-TCCGTTCCACTTGATCACAA, UboxMR-GCCGAACCATTGTCACTTTT. Resistance to bacterial blight was measured as lesion length (cM) and indicated at the bottom of each lane. All resistant lines are homozygous for the mutant allele, whereas the susceptible lines include both heterozygous and homozygous genotypes.