(a) Schematic view of the sequencing strategy. Based on the O. barthii physical map, nine FPC contigs span the ~18-Mb short arm of Chr3. A minimum tiling path of BACs across the FPC contigs was divided into six regions (each approximately 3 Mb) from which six pools of BACs were created. Each pool was sequenced and assembled to create contigs and scaffolds. Superscaffolds across pool boundaries were generated using the BAC end sequence information. (b) Morphology of a mature O. barthii plant. (c) Seed morphology of O. barthii showing grains and spikelets with awns.