Fig. 5

The intermediate metabolites and gene expression levels in the TCA cycle in developing seeds of WT, phs39, and Osaah mutants. A‒H Contents of acetyl-CoA (A), citrate (B), isocitrate (C), aconitate (D), succinate (E), α-ketoglutarate (F), ADP (G) and ATP (H) in 20 and 25 DAP seeds of WT, phs39 and Osaah mutants. I The expression levels of genes related to the TCA cycle in 20 DAP seeds. All values are means ± SD (n = 3). Asterisks indicate statistically significant differences compared with WT by Student’s t-test (*P < 0.05; **P < 0.01)