Fig. 2

The expression pattern of OsNOMT and sakuranetin content in different rice tissues. A GUS staining of 7-day-old seedlings of pOsNOMT::GUS. Scale bar = 1 cm. B GUS staining of 20-day-old leaf (top) and leaf sheath (bottom) of pOsNOMT::GUS. Scale bar = 1 mm. C GUS staining of pOsNOMT::GUS seeds at 15 DAF. The top is the intact husked seeds, and the bottom is the cross section of the seeds (the left half contains the ridge of the seed, the right half does not). Scale bar = 0.5 mm. D qRT-PCR analysis of the expression levels of OsNOMT in different tissues of ZH11. E LC–MS/MS analysis of the sakuranetin content in different tissues of ZH11. Different letters indicate significant differences at P < 0.05 as determined by one-way ANOVA with Tukey’s test (mean ± s.d., n = 3, individual values and means are shown, biologically independent samples) (D, E). n.d., not detected