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Rice
Fig. 5 | Rice

Fig. 5

From: Heat Stress Responsive Aux/IAA Protein, OsIAA29 Regulates Grain Filling Through OsARF17 Mediated Auxin Signaling Pathway

Fig. 5

OsIAA29 competes with OsIAA21 in the binding to OsARF17. A The expression of OsIAA29 in developing seeds of ZH11 under high temperature and normal temperature. B Western-Blot detects the GFP tagged OsIAA29 in OE29; vector is pZmUbi::OsIAA29-GFP. C Dual luciferase analysis of the effect of auxin to OsIAA21. D MS analysis of changes in auxin content in rice seeds under high temperature/normal temperature conditions. E Immunoblot of pull-down/protein competition assay OsARF17-His and OsIAA29-GST with increasing amounts of OsIAA21-MYC. The anti‐His antibody is indicated pull-down at the top panel, while the input shown at the bottom panel. F Yeast three-hybrid assay analyzing the OsIAA21-OsARF17 interaction in the presence or absence of co-expressed OsIAA29. Co-transformants were spotted on 2-SD (-Leu-Trp) medium to check for viability and on 4-SD (-Met-His-Leu-Trp) medium to test the interaction and competition. Data are mean standard error (SE) for three replicates. *P < 0.05, **P < 0.01. P-values produced by two-tailed Student’s t-test

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