Fig. 2

Phenotypic analysis of OsIAA29 CRISPR lines (osiaa29) and overexpression plants (OE29). A Mutation sites in osiaa29-1 and osiaa29-2, as compared with wild-type (WT) sequences, protospacer-adjacent motif sequences are shown in bold, and inserted or deleted nucleotides are indicated in red. B Relative expression level of overexpression materials lines in ZH11. Data are mean standard error (SE) for three replicates. *P < 0.05, **P < 0.01. P-values produced by two-tailed Student’s t-test. C Detection of eGFP fusion proteins in ZH11 and OsIAA29 overexpression line (OE29-1). Total proteins extracted from developing caryopses at 7-DAP were used for western blot analysis with an anti-GFP antibody. D the appearance of osiaa29-1 did not change under normal conditions. E the shrunken seeds osiaa29-1 material in the filling stage under high temperature conditions. Seeds at 3, 5, 7, 10, 14, and 21 DAF were observed. Scale bars are 1 mm. F images of the seeds longitudinal section the in different periods and stained with toluidine blue. G The WT, oaiaa29-1, and OE29-1 images of 200 grains of mature seeds; scale bars are 10 mm. And cross-sections of the seeds; scale bars are 1 mm. H SEM images of the mature endosperm at different parts: the areas are indicated by the squares in (c-c1). Scale bars are 5 mm (a-a1); 2 mm (b-b1); 2 mm (c-c1); 10 μm (d-f1)