Fig. 1

Sequence and expression pattern of OsIAA29. A The structure of OsIAA29. The blue boxes represent the exons, the black lines represent the intron and the white boxes the UTRs. B Schematic diagram of the domains of OsIAA29. C Compared with other canonical AUX/IAA, OsIAA29 lacks I and II domains. D qRT-PCR analysis of OsIAA29 in different tissues. E In situ hybridization of sectioned caryopses collected at 3, 5, 7, 10, and 14 DAP (day after pollination) using antisense and sense probes. OsIAA29 was highly expressed in the seed embryo layer and the starchy endosperm. Em, Embryo; Ep, endopleura; AL, Aleurone, scale bars are 100 µm. F Histochemical GUS activity staining using proOsIAA29::GUS, scale bars are 1 mm