Fig. 5

AvrPik-D enhances WG7’s transcriptional activity. A qRT-PCR detected the expression of WG7 in the leaves of AvrPik-D overexpressed lines. Data were normalized to the expression level of OsActin1. Data are shown as mean ± SEM (Different letter indicate a significant difference, P < 0.05, n = 3). B Constructs used in the transcriptional activity assays. Coding regions of WG7 and WG7¹⁻⁶⁹⁸ were fused with GAL4BD under the control of the CaMV 35S promoter. The Firefly luciferase gene driven by four tandem copies of the GAL4 DNA binding site fused immediately upstream of four tandem copies of the constitutive D1-3 element (GAL4(4X)-D1-3(4X)), and the Renilla luciferase gene driven by the CaMV 35S promoter was used as the reporter and internal control, respectively. C The transcriptional activity assay of WG7 and WG7¹⁻⁶⁹⁸ in rice protoplasts. HOS15, a transcription suppressor, and ARF5M, a transcription activator, were used as the controls. Asterisks indicate a significant difference between the empty vector with WG7 and WG7¹⁻⁶⁹⁸ (Student’s t-test, **P < 0.01, ****P < 0.0001, n = 3)