Fig. 3

WG7 negatively regulates the basal resistance of rice against M. oryzae. A, B Disease symptoms of wg7 and wild type (WT) plants after punch inoculation with M. oryzae strains FJ802 (without AvrPik-D) and FJ802^AvrPik-D (with AvrPik-D). The images were photographed at 10 days post inoculation (dpi). Scale bar = 0.5 cm. Lesion length was measured using ImageJ software. Data are shown as mean ± standard error (SD) (Different letter indicate P < 0.05, n ≥ 14). Relative fungal biomass of M. oryzae in inoculated leaves, as quantified by qPCR comparing the DNA amounts of the fungal gene MoPot2 and the rice gene OsUG. Data are shown as mean ± SD (Different letter indicate a significant difference P < 0.05, n = 9). Experiments were repeated three times with similar results. E and F Spray and punch inoculation of WG7 overexpression lines and wild type (WT) plants with M. oryzae strain FJ802^AvrPik-D (with AvrPik-D). Scale bars = 0.5 cm. Relative fungal biomass of M. oryzae in inoculated leaves, as quantified by qPCR comparing the DNA amounts of the fungal gene MoPot2 and the rice gene OsUG. Data are shown as mean ± SD (Different letter indicate a significant difference P < 0.05). C, D and G, H Chitin-induced ROS burst in wg7, WG7 overexpression and wild type (WT) plants. C and G Leaf disks were treated with water or 8 nM chitin, and ROS accumulation was measured by luminol assay. Data are shown as mean ± SD (n ≥ 6). D and H Calculate the maximum ROS value for each individual leaf disk. Data are shown as mean ± SD (Different letter indicate a significant difference P < 0.05, n ≥ 6). I Expression of OsWRKY45 and OsMT2b in the leaves of wg7, WG7 overexpression lines and wild type (WT) plants under normal conditions. Data were normalized to the expression level of OsActin1. Data are shown as mean ± SEM (The letters a to d indicate a significant difference P < 0.05, n = 3)