Table 2 The list of polymorphic markers along with their sequence, chromosome number, annealing temperature and range of amplified product size
S.No. | Marker | Sequence (5’-3’) | Chromosome number | Annealing Temperature | Amplified product size (bp) |
|---|---|---|---|---|---|
1 | RM1 | F: GCGAAAACACAATGCAAAAA R: GCGTTGGTTGGACCTGAC | 1 | 55 | 90–115 |
2 | RM31 | F: CGCTCCTCCACTCTTCTCCTACC R: CGTGCAGAAAGTCCATTACTCTCC | 5 | 55 | 140–180 |
3 | RM44 | F: ACGGGCAATCCGAACAACC R: CGAGGATGGTTGTTCACTTG | 8 | 55 | 99–105 |
4 | RM205 | F: CTGGTTCTGTATGGGAGCAG R: CTGGCCCTTCACGTTTCAGTG | 9 | 55 | 230–250 |
5 | RM208 | F: TCTGCAAGCCTTGTCTGATG R: TAAGTCGATCATTGTGTGGACC | 2 | 55 | 173–290 |
6 | RM216 | F: GCATGGCCGATGGTAAAG R: GGATTTTCTGATAGCGGTAA | 10 | 55 | 140–160 |
7 | RM228 | F: CTGGCCATTAGTCCTTGG R: GCTTGCGGCTCTGCTTAC | 10 | 55 | 100–160 |
8 | RM232 | F: CCGGTATCCTTCGATATTGC R: CCGACTTTTCCTCCTGACG | 3 | 55 | 158–190 |
9 | RM258 | F: TGCTGTATGTAGCTCGCACC R: TGGCCTTTAAAGCTGTCGC | 10 | 55 | 150–290 |
10 | RM267 | F: TGCAGACATAGAGAAGGAAGTG R: AGCAACAGCACAACTTGATG | 5 | 55 | 156–175 |
11 | RM313 | F: GAGGTACTTCCTCCGTTTCAC R: AGTCAGCTCACTGTGCAGTG | 1 | 55 | 111–160 |
12 | RM315 | F: GAGGTACTTCCTCCGTTTCAC R: AGTCAGCTCACTGTGCAGTG | 1 | 55 | 105–140 |
13 | RM443 | F: GATGGTTTTCATCGGCTACG R: AGTCCCAGAATGTCGTTTCG | 1 | 55 | 100–124 |
14 | RM445 | F: CGTAACATGCATATCACGCC R: ATATGCCGATATGCGTAGCC | 7 | 55 | 200–295 |
15 | RM461 | F: GGACATACGTACAGTGGTTGATGC R: GTGCATCAAACGACAAACTCTCC | 6 | 55 | 190–200 |
16 | RM471 | F: ACGCACAAGCAGATGATGAG R: GGGAGAAGACGAATGTTTGC | 4 | 55 | 100–106 |
17 | RM474 | F: AAGATGTACGGGTGGCATTC R: TATGAGCTGGTGAGCAATGG | 10 | 55 | 205–275 |
18 | RM481 | F: TAGCTAGCCGATTGAATGGC R: TAGCTAGCCGATTGAATGGC | 7 | 55 | 150–197 |
19 | RM514 | F: AGATTGATCTCCCATTCCCC R: CACGAGCATATTACTAGTGG | 3 | 55 | 220–280 |
20 | RM555 | F: TTGGATCAGCCAAAGGAGAC R: CCTGTACGTTGATCCGAAGC | 2 | 55 | 215–223 |
21 | RMS-SF21 − 5 | F: GAGTTGGGGGTCGAGAAATC R: CGTACGTGCGGCTAGGATCAA | 1 | 55 | 135–200 |
22 | RM1108 | F: GCTCGCGAATCAATCCAC R: GCTGGATCACAGATCATTGC | 10 | 55 | 124–142 |
23 | RM3317 | F: AGCAACCTGACAGAAGAATG R: TCTCGTTGAGTTGGAAGAAG | 4 | 55 | 155–180 |
24 | RM3530 | F: GTAGATCCGGTCAGCTCCTC R: CAAGGAGATTCCCTTCCATG | 1 | 55 | 160–195 |
25 | RM5359 | F: CGTGATCTCGTGCATCCC R: CCCTCAGGAGCTTCATGAAC | 1 | 55 | 175–200 |
26 | RM6344 | F: ACACGCCATGGATGATGAC R: TGGCATCATCACTTCCTCAC | 7 | 50 | 100–120 |
27 | RM6869 | F: GAGCTCCTTGTAGTGACCCG R: ATCAGCCTCGCCAGCTTC | 12 | 61 | 126–140 |
28 | RM10318 | F: TGTCTCACACATTGCACACTTACC R: GGCCTAACCCAACACATGTCC | 1 | 55 | 175–196 |
29 | RMS-PPR9 − 1 | F: GAGTTTTGAATAGATTTACGTGTGGA R: AGTGTCCAGATTCGTAGTAATGC | 10 | 58 | 114–168 |
30 | DRRMRf3 10 | F: GATGGCAAGCTTCAGAACA R: CTAATTCTGGGCGAGCAAAG | 1 | 55 | 210–250 |