Fig. 5
From: Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene
Construction of the female sterility maintainer system. a, Diagram of the two T-DNA cassettes in GSX-H569-Red vector. White arrows represent promoters; light blue and red boxes represent genic regions; black boxes represent terminators; and 35Se represents 35S enhancer element. b, Diagram showing the T-DNA insertion site in HR1607-2B maintainer genome. Red triangle indicates the insertion site. Double arrows represent the two genes flanking the T-DNA insertion site. The numbers under the line represent the length between the T-DNA insertion site to the two flanking genes. c, PCR examination of the transgene. Primers for PCR are as shown in Fig. 5a. #1 and #2 are positive transgenic plants, #3 and #4 are negative transgenic plants, and nc is negative control plant. d, Sequence of TAIL PCR products showing the T-DNA insertion site sequence. Sequences marked in black are rice genome sequences flanking the T-DNA insertion site. Sequences marked in red shows the 21 bp right border sequence to the MEL2 promoter element. Sequence marked in green shows the 76 bp left border sequence to PIN ΙΙ element. The right border and left border are underlined in black and red respectively. e, PCR verification of the T-DNA insertion site. Primers for PCR are as shown in Fig. 5a. #A indicates HR1607-2A; #B indicates HR1607-2B. f, HR1607-2A and HR1607-2B plants at mature stage; bar = 5 cm. g, HR1607-2B panicle under bright field (left) and a red fluorescence filter (right); bar = 1 cm. h, I2-KI staining of the pollen grains of HR1607-2A and HR1607-2B plants; bar = 100 μm. i, Rates of fertile and infertile pollen, fluorescent and non-fluorescent seeds produced by HR1607-2B plants. j, Yield performance of the F1 hybrids. The parental lines for these hybrids are shown in X axis