Fig. 4
From: Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene
Verification of the mutant gene. a–d, Complementation of h569 mutant. Com + and Com– represent plants with and without the transgene, respectively. e–h, CRISPR knockout of MEL2 gene in WYG. WT WYG and CRISPR mutant in WYG background (cr-rrm) were shown. Seed-setting (a, e), panicles (b, f), I2-KI stained pollen grains (c, g), and morphology of embryo sac at mature stage (d, h) were shown. Bars: a, e = 5 cm; b, f = 1 cm; c, g = 100 μm; d, h = 20 μm