Fig. 3
From: Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene
Mutant gene cloning and conserved domain analysis. a, Determination of the mutation site. The top section shows the distribution of Euclidean distance (ED) scores of SNP sites along chromosomes in h569 mutant. MEL2 (LOC_Os12g38460) gene structure with the mutation site in h569 and the mutation site in the CRISPR knockout mutant cr-rrm are shown underneath. White boxes represent 5′- and 3′-UTRs, black boxes represent coding sequence, and the lines between boxes represent introns. The genomic fragment for gene complementation is shown by the double-headed arrow. b, Conserved domain analysis of MEL2 protein. Red arrow indicates the amino acid substitution site in the mutant protein in h569