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Rice
Fig. 5 | Rice

Fig. 5

From: YGL3 Encoding an IPP and DMAPP Synthase Interacts with OsPIL11 to Regulate Chloroplast Development in Rice

Fig. 5

OsPIL11 directly binds to the YGL3 promoter. (a) Schematic showing the localization of the PBE-box in the YGL3 promoter. (b) Transcriptional activation activities of OsPILs on YGL3 promoters in rice protoplasts. Relative LUC activity was calculated as LUC/REN. (c) Yeast one-hybrid assay showed that OsPIL11 can bind to the YGL3 promoter. (d and e) EMSA assay showed that OsPIL11 can directly bind to the promoter of YGL3. Ten- and 20-fold excesses of non-labeled probes were used for competition. Asterisks indicate statistical significance as determined by Student’s t-test (**P < 0.01; 0.01 < *P < 0.05)

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