Fig. 2

Map-based cloning of YGL3. (a) Mapping of the YGL3 locus in a 122-kb region on Chromosome 3 (Chr3). (b) Phenotypic characteristics of complementation plants (Com-1 and Com-2) of ygl3 at 28℃. Scale bar = 2 cm. (c) Phenotypic characteristics of knockout plants (CP-1 and CP-2) of YGL3 at 28℃. Scale bar = 2 cm. (d) Chloroplast ultrastructures of WT, ygl3, complementation (Com-1), and knockout transgenic plants (CP-1) at 28℃. (e and f) Chlorophyll contents of WT, ygl3, complementation plants, and knockout plants. (g and h) Transcriptional levels of YGL3 in complementation and knockout transgenic plants. The RNA was extracted from two-week-old plants. The rice Actin gene was used as the internal control. (i and j) Immunoblot analysis of YGL3 protein in WT, ygl3, and transgenic plants at 28℃. Total protein was extracted from two-week-old plants. β-Actin was used as a loading control. Data are shown as mean ± standard deviation from three biological replicates. Asterisks indicate statistical significance as determined by Student’s t-test (**P < 0.01; 0.01 < *P < 0.05)