Fig. 3

Characterization of OsBAG6 overexpression lines under saline-alkaline stress conditions. Image (A) and survival rates (B) of Kitaake and 3 independent OsBAG6OE lines before and after recovery from saline-alkaline stress condition (25 mM Na₂CO₃, pH = 10.0). In A, scale bar = 8 cm. In B, Data represent mean ± SD of 5 replicates (n = 5, 32 plants per genotype were used to calculate survival rate per replicate). Significant differences were evaluated by two-way ANOVA, followed by Tukey’s multiple comparison test. C DAB staining of leaves of indicated genotypes before and after 24 h saline-alkaline stress (25 mM Na₂CO₃, pH = 10.0). Three biological replicates were performed, with 25 plants per treatment