Fig. 6

Mutant mpk7/14 showed enhanced ABA response. A The germination performance of ZH11 and mpk7/14. The germinated seeds were counted after 6 days of ABA treatment, Values represent the mean ± SD of three biological replicates (30 seeds for each replicate). The asterisks indicate significant differences compared with ZH11. Student’s t-tests were used to generate p values (*p < 0.05, **p < 0.01). B Phenotypes of ZH11and mpk7/14 after 6 days of ABA treatment on 1/2 MS culture medium without or with ABA (0.2 μM or 2 μM) (Scale bar = 1 cm). C The lengths of shoots and roots of ZH11 and mpk7/14 after 6 days of ABA treatment. Values represent the mean ± SD of three biological replicates (10 germinated seeds for each replicate). The asterisks indicate significant differences compared with mock treatment. Student’s t-tests were used to generate p values (**p < 0.01). D Expression analysis of ABA responsive genes in ZH11 and mpk7/14. Seeds were soaked in water for six hours at 30 °C and ten embryos were cut for gene expression analysis. Values represent the mean ± SD of three biological replicates. The asterisks indicate significant differences compared with ZH11. Student’s t-tests were used to generate p values (*p < 0.05, **p < 0.01)