Fig. 2

Expression characteristics of OsMKKK62, OsMKK3 and OsMPK7. A Transcription analysis of OsMKKK62, OsMKK3 and OsMPK7 in different tissues by quantitative RT-PCR. Shoot and root were sampled at three-leaf stage; young panicle, first leaf, stem and mature root were sampled before heading; embryos were sampled at the late period of dough stage. Values represent the means ± SD of three biological replicates. B Subcellular localization of OsMKK3 and OsMPK7 in rice protoplasts. GFP, OsMKK3-GFP and GFP-OsMPK7 driven by the 35S promoter under green fluorescence, red fluorescence, bright field, and merged views (Scale bar = 10 μm). C, D The expression of OsMKK3 (C) and OsMPK7 (D) in embryos treated with ABA or GA. The seeds were treated with 100 μM ABA, 100 μM GA and water respectively. After 3, 6, 12, and 24 h of treatment, ten embryos were cut for gene expression analysis. Values represent the mean ± SD of three biological replicates. Student’s t-tests were used to generate p values. (* = p < 0.05, ** = p < 0.01)