Fig. 5
From: Identification of NADPH Oxidase Genes Crucial for Rice Multiple Disease Resistance and Yield Traits
Mutations of RbohA, RbohB, RbohC, RbohE, RbohH, and RbohI suppress rice ROS production. (A, B) chitin- and flg22-induced reactive oxygen species (ROS) bursts in the leaves of the Rboh mutant lines and the TaPei309 (TP309) control. Data are shown as mean ± SD (n = 4). All the P values were determined by One-way ANOVA analysis, and the colored dots associated with the bars indicate the value of biologically independent samples. (C) O•2− and H2O2 accumulation in the indicated mutants and the TP309 control at 48 h post-inoculation with M. oryzae. Nitrotetrazolium Blue chloride (NBT) was used as an indicator of O•2− amounts (blue). 3, 3 N-diaminobezidin (DAB) was used as an indicator of H2O2 amounts (reddish-brown). Scale bars = 5 mm