Fig. 5

PG activity and structural analysis of cell wall in hcc1. A Changes of PG activity in the leaf of WT and hcc1 at the seedling stage (Ss), tillering stage (Ts) and mature stage (Ms). B Comparison of total pectin content in the leaf blade between WT and hcc1 at Ms. C Comparison of D-GA content in the leaves between WT and hcc1 at Ms. D TEM showing the subcellular structure of leaf of WT and E hcc1 at Ms. Where C, chloroplast; P, plastoglobule; S, starch grain; and W, cell wall. Scale bar in (D, E) = 1 μm. F Comparison of cell wall thickness of WT and hcc1 in the leaf at Ms. Mean and SD were obtained from three independent measurements (A–C). The values in (F) are the mean ± SD of 10 different determinations (red word in images of D and E). Statistical analysis was performed using two-tailed Student’s t-test, *, ** and **** indicate p < 0.05, p < 0.01 p < 0.0001 and respectively