Fig. 4

Map-based cloning of the WLP3 gene. A Preliminary mapping of the wlp3 mutant using 21 recessive F₂ plants with 268 SSR markers. The gene mapped to a region between molecular markers B3-22 to B3-23 on chromosome 3. B-C Fine mapping of WLP3 further localized the mutation to the 56 kb genomic region between M4 and M5. D WLP3 gene structure. Red arrows indicate mutation site. E–F Mutation sites. G Functional complementation of wlp3. Transgenic plants were verified by the presence of the hygromycin selectable marker gene. H Chlorophyll content of complementary lines