Fig. 9
From: Rice Basic Helix-Loop-Helix 079 (OsbHLH079) Delays Leaf Senescence by Attenuating ABA Signaling
OsbHLH079 represses the transcription of OsABF2, OsABF4, OsABI5, and OsNAP. A Description of the constructs used in the dual-luciferase reporter assay, including the effectors, reporters, and internal control. LUC was fused to proOsABF2 (-1,553 to -1), proOsABF4 (-1,709 to + 7), proOsABI5 (-1,971 to + 58), or proOsNAP (-1,542 to + 84). LUC, luciferase; NOS, NOS terminator; pUbi, promoter of Ubiquitin; Rluc, renilla luciferase. B The repression of proOsABF2, proOsABF4, proOsABI5, and proOsNAP by OsbHLH079-MYC expression in the dual-luciferase reporter assay. MYC was used as a negative control. The LUC activity of each sample was represented relative to that of the negative control, set as 1. Averages and standard deviations were obtained from five independent samples. Significant differences between means were determined using a two-tailed Student’s t-test (**P < 0.01, and ***P < 0.001). LUC, luciferase; pUbi, promoter of Ubiquitin