Fig. 8

Transcriptional analysis of genes involved in ABA signaling during natural leaf senescence in rice. A-F Relative transcript abundances of OsABF2 (A), OsABF4 (B), OsABI5 (C), OsbZIP23 (D), OsEEL (E), and OsNAP (F) were measured in flag leaves of WT, osbhlh079-D, OsbHLH079-OE, osbhlh079-1, and osbhlh079-2. Total RNAs isolated from the samples shown in Fig. 5 were subjected to RT-qPCR analysis. The mRNA levels of GAPDH, a reference gene, were used to normalize. The values shown in the graphs are averages of four independent samples (around 5 flag leaves per sample), and the error bars represent standard deviations. Differences between the means were statistically analyzed using two-tailed Student’s t-test (*P < 0.05, **P < 0.01, and ***P < 0.001). These analyses were repeated twice with similar results. DAH, days after heading; NS, not significant